cell cycle assay au565 Search Results


au565  (ATCC)
96
ATCC au565
Effects of 2-APB on breast cancer cell lines. ( A ) Cell viability. The 24-h treatment with 2-APB (50–200 µM) significantly suppressed viability in all three cell lines except for 50 µM 2-APB on MDA-MB-231. ( B – E ) show data for cells exposed to 200 µM 2-APB for 24 h. ( B ) TRPM7 mRNA levels. 2-APB did not significantly affect TRPM7 mRNA expression. ( C ) LDH release. 2-APB had a significant effect on the LDH of MDA-MB-231 only. ( D ) Apoptosis. 2-APB had a significant effect on the apoptosis of MDA-MB-231 only. ( E ) 2-APB caused an increase in the percentage of cells in the S phase and a corresponding decrease in cells in the G0/G1 phase in all three cell lines and also caused a decrease in the number of cells in the G2/M phase in <t>AU565</t> and T47D. ( F ) Representative images from the cell cycle assay of breast cancer cell lines. Significant differences ( p < 0.05) from control are indicated by “*”.
Au565, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effects of 2-APB on breast cancer cell lines. ( A ) Cell viability. The 24-h treatment with 2-APB (50–200 µM) significantly suppressed viability in all three cell lines except for 50 µM 2-APB on MDA-MB-231. ( B – E ) show data for cells exposed to 200 µM 2-APB for 24 h. ( B ) TRPM7 mRNA levels. 2-APB did not significantly affect TRPM7 mRNA expression. ( C ) LDH release. 2-APB had a significant effect on the LDH of MDA-MB-231 only. ( D ) Apoptosis. 2-APB had a significant effect on the apoptosis of MDA-MB-231 only. ( E ) 2-APB caused an increase in the percentage of cells in the S phase and a corresponding decrease in cells in the G0/G1 phase in all three cell lines and also caused a decrease in the number of cells in the G2/M phase in AU565 and T47D. ( F ) Representative images from the cell cycle assay of breast cancer cell lines. Significant differences ( p < 0.05) from control are indicated by “*”.

Journal: Cancers

Article Title: The Role of Transient Receptor Potential Melastatin 7 (TRPM7) in Cell Viability: A Potential Target to Suppress Breast Cancer Cell Cycle

doi: 10.3390/cancers12010131

Figure Lengend Snippet: Effects of 2-APB on breast cancer cell lines. ( A ) Cell viability. The 24-h treatment with 2-APB (50–200 µM) significantly suppressed viability in all three cell lines except for 50 µM 2-APB on MDA-MB-231. ( B – E ) show data for cells exposed to 200 µM 2-APB for 24 h. ( B ) TRPM7 mRNA levels. 2-APB did not significantly affect TRPM7 mRNA expression. ( C ) LDH release. 2-APB had a significant effect on the LDH of MDA-MB-231 only. ( D ) Apoptosis. 2-APB had a significant effect on the apoptosis of MDA-MB-231 only. ( E ) 2-APB caused an increase in the percentage of cells in the S phase and a corresponding decrease in cells in the G0/G1 phase in all three cell lines and also caused a decrease in the number of cells in the G2/M phase in AU565 and T47D. ( F ) Representative images from the cell cycle assay of breast cancer cell lines. Significant differences ( p < 0.05) from control are indicated by “*”.

Article Snippet: Human kidney embryo cell line HEK293 (WT-HEK), breast cancer cell line MDA-MB-231 (WT-231), AU565, and T47D were obtained from ATCC ® (Manassas, VA, USA).

Techniques: Expressing, Cell Cycle Assay, Control